Synthesis of 5-Fluorouracil Polymer Conjugate and ¹⁹F NMR Analysis of Drug Release for MRI Monitoring.

ABSTRACT: To monitor the release of fluorinated drugs from polymeric carriers, a novel ¹⁹F MRI enzyme-responsive contrast agent was developed and tested. This contrast agent was prepared by conjugation of 5-fluorouracil (5-FU) to hyperbranched poly(N,N-dimethylacrylamide) (HB-PDMA) via an enzyme-degradable peptide linker. Due to the different molecular sizes, the release of 5-FU from the 5-FU polymer conjugate resulted in a sufficiently substantial difference in spin-spin T₂¹⁹F NMR/MRI relaxation time that enabled differentiating between attached and released drug states. The 5-FU polymer conjugate exhibited a broad signal and short T₂ relaxation time under ¹⁹F NMR analysis. Incubation with the enzyme induced the rele

SUBMITTER: Alhaidari LM 

PROVIDER: S-EPMC10097235 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature