Light Stimulation of Neurons on Organic Photocapacitors Induces Action Potentials with Millisecond Precision.

ABSTRACT: Nongenetic optical control of neurons is a powerful technique to study and manipulate the function of the nervous system. This research has benchmarked the performance of organic electrolytic photocapacitor (OEPC) optoelectronic stimulators at the level of single mammalian cells: human embryonic kidney (HEK) cells with heterologously expressed voltage-gated K⁺ channels and hippocampal primary neurons. OEPCs act as extracellular stimulation electrodes driven by deep red light. The electrophysiological recordings show that millisecond light stimulation of OEPC shifts conductance-voltage plots of voltage-gated K⁺ channels by ≈30 mV. Models are described both for understanding the experimental findings at the level of K⁺ channel kinetics in HEK cells, as

SUBMITTER: Schmidt T 

PROVIDER: S-EPMC10097427 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature