Mapping the Response of Human Osteocytes in Native Matrix to Mechanical Loading Using RNA Sequencing.

ABSTRACT: Osteocytes sense mechanical loads and transduce mechanical signals into a chemical response. They are the most abundant bone cells deeply embedded in mineralized bone matrix, which affects their regulatory activity in the mechanical adaptation of bone. The specific location in the calcified bone matrix hinders studies on osteocytes in the in vivo setting. Recently, we developed a three-dimensional mechanical loading model of human osteocytes in their native matrix, allowing to study osteocyte mechanoresponsive target gene expression in vitro. Here we aimed to identify differentially expressed genes by mapping the response of human primary osteocytes in their native matrix to mechanical loading using RNA sequencing. Human fibular bone was retrieved from 10 donors (age: 32-82 years, 5 female

SUBMITTER: Zhang C 

PROVIDER: S-EPMC10097643 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature