Actively transcribed rDNA and distal junction (DJ) sequence are involved in association of NORs with nucleoli.

ABSTRACT: Although they are organelles without a limiting membrane, nucleoli have an exclusive structure, built upon the rDNA-rich acrocentric short arms of five human chromosomes (nucleolar organizer regions or NORs). This has raised the question: what are the structural features of a chromosome required for its inclusion in a nucleolus? Previous work has suggested that sequences adjacent to the tandemly repeated rDNA repeat units (DJ, distal junction sequence) may be involved, and we have extended such studies by addressing several issues related to the requirements for the association of NORs with nucleoli. We exploited both a set of somatic cell hybrids containing individual human acrocentric chromosomes and a set of Human Artificial Chromosomes (HACs) carrying different parts of a NOR, includin

SUBMITTER: Liskovykh M 

PROVIDER: S-EPMC10097779 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature