Identification of nicotine-seeking and avoiding larval zebrafish using a new three-choice behavioral assay.

ABSTRACT:

Introduction

Nicotine dependence is one of the main causes of preventable diseases in the United States. Nicotine-seeking and avoidance behavioral assays in larval zebrafish could be used for identifying potential new pharmacotherapeutics in an early phase of drug discovery and could facilitate the identification of genes and genomic variations associated with nicotine-seeking and avoidance behavior.

Methods

A new three-choice behavioral assay has been developed for the identification of nicotine-seeking and avoiding larval zebrafish. The three choices are represented by three compartments of a gradient maze. Video-recording and subsequent quantitative analysis of the swimming track was carried out using EthovisionXT (Noldus).

Results

Three behavioral phenotypes could

SUBMITTER: Schneider H 

PROVIDER: S-EPMC10098024 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature