Clostridioides difficile minimal nutrient requirements for flagellar motility.

ABSTRACT: As many gastro-intestinal pathogens, the majority of Clostridioides difficile strains express flagella together with a complete chemotaxis system. The resulting swimming motility is likely contributing to the colonization success of this important pathogen. In contrast to the well investigated general energy metabolism of C. difficile, little is known about the metabolic requirements for maintaining the ion motive force across the membrane, which in turn powers the flagellar motor. We studied here systematically the effect of various amino acids and carbohydrates on the swimming velocity of C. difficile using video microscopy in conjunction with a software based quantification of the swimming speed. Removal of individual amino acids from the medium identified proline a

SUBMITTER: Schwanbeck J 

PROVIDER: S-EPMC10098170 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature