Effects of assisted reproductive technology on gene expression in heart and spleen tissues of adult offspring mouse.

ABSTRACT:

Objectives

Assisted reproductive technology (ART) is an important part of reproductive medicine, whose possible effects on offspring's health have drawn widespread attention in recent years. However, relevant studies are limited to postnatal short-term follow-up and lack of diverse sample sources analysis other than blood.

Methods

In this study, a mouse model was used to explore the effects of ART on fetal development and gene expression in the organs of offspring in the adulthood using next-generation sequencing. The sequencing results were then analyzed.

Results

The results showed that it caused abnormal expression in 1060 genes and 179 genes in the heart and spleen, respectively. Differentially expressed genes (DEGs) in the heart are mainly enriched in RNA synthesi

SUBMITTER: Chen H 

PROVIDER: S-EPMC10098333 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature