Degradation of PET Bottles by an Engineered Ideonella sakaiensis PETase.

ABSTRACT: Extensive plastic production has become a serious environmental and health problem due to the lack of efficient treatment of plastic waste. Polyethylene terephthalate (PET) is one of the most used polymers and is accumulating in landfills or elsewhere in nature at alarming rates. In recent years, enzymatic degradation of PET by Ideonella sakaiensis PETase (IsPETase), a cutinase-like enzyme, has emerged as a promising strategy to completely depolymerize this polymer into its building blocks. Here, inspired by the architecture of cutinases and lipases homologous to IsPETase and using 3D structure information of the enzyme, we rationally designed three mutations in IsPETase active site for enhancing its PET-degrading activity. In particular, the S238Y mutant, locat

SUBMITTER: Sevilla ME 

PROVIDER: S-EPMC10098701 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature