Bacterial Colony Phenotyping with Hyperspectral Elastic Light Scattering Patterns.

ABSTRACT: The elastic light-scatter (ELS) technique, which detects and discriminates microbial organisms based on the light-scatter pattern of their colonies, has demonstrated excellent classification accuracy in pathogen screening tasks. The implementation of the multispectral approach has brought further advantages and motivated the design and validation of a hyperspectral elastic light-scatter phenotyping instrument (HESPI). The newly developed instrument consists of a supercontinuum (SC) laser and an acousto-optic tunable filter (AOTF). The use of these two components provided a broad spectrum of excitation light and a rapid selection of the wavelength of interest, which enables the collection of multiple spectral patterns for each colony instead of relying on single band analysis. The performan

SUBMITTER: Doh IJ 

PROVIDER: S-EPMC10098818 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature