High-Performance Detection of Exosomes Based on Synergistic Amplification of Amino-Functionalized Fe₃O₄ Nanoparticles and Two-Dimensional MXene Nanosheets.

ABSTRACT: Exosomes derived from cancer cells have been recognized as a promising biomarker for minimally invasive liquid biopsy. Herein, a novel sandwich-type biosensor was fabricated for highly sensitive detection of exosomes. Amino-functionalized Fe₃O₄ nanoparticles were synthesized as a sensing interface with a large surface area and rapid enrichment capacity, while two-dimensional MXene nanosheets were used as signal amplifiers with excellent electrical properties. Specifically, CD63 aptamer attached Fe₃O₄ nanoprobes capture the target exosomes. MXene nanosheets modified with epithelial cell adhesion molecule (EpCAM) aptamer were tethered on the electrode surface to enhance the quantification of exosomes captured with the detection of remaining protein

SUBMITTER: Zhuang L 

PROVIDER: S-EPMC10099274 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature