Complete meiosis in rat prepubertal testicular tissue under in vitro sequential culture conditions.

ABSTRACT:

Background

Testicular tissue cryopreservation before gonadotoxic treatments allows fertility preservation in children suffering from cancer. Fertility restoration strategies, in particular in vitro maturation of prepubertal testicular tissue, are being developed mainly in animal models. The rat, widely used in biomedical research, including in reproductive biology, is a relevant model.

Objectives

To determine whether sequential two-step culture protocols can improve the efficiency of rat in vitro spermatogenesis.

Materials and methods

Rat prepubertal testicular tissues were cultured on agarose gels with either a one-step or two-step protocol with or without polydimethylsiloxane (PDMS) ceiling chips. The progression of spermatogenesis, germ/Sertoli cell ratio, cell pro

SUBMITTER: Saulnier J 

PROVIDER: S-EPMC10099474 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature