Responses of Salmonella biofilms to oxidizing biocides: Evidence of spatial clustering.

ABSTRACT: The spatial organization of biofilm bacterial communities can be influenced by several factors, including growth conditions and challenge with antimicrobials. Differential survival of clusters of cells within biofilms has been observed. In this work, we present a variety of methods to identify, quantify and statistically analyse clusters of live cells from images of two Salmonella strains with differential biofilm forming capacity exposed to three oxidizing biocides. With a support vector machine approach, we showed spatial separation between the two strains, and, using statistical testing and high-performance computing (HPC), we determined conditions which possess an inherent cluster structure. Our results indicate that there is a relationship between biocide potency and inherent biofilm

SUBMITTER: Guest K 

PROVIDER: S-EPMC10099496 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature