Immobilization and Application of Fatty Acid Photodecarboxylase in Deep Eutectic Solvents.

ABSTRACT: Since its discovery in 2017, the fatty acid decarboxylase (FAP) photoenzyme has been the focus of extensive research, given its ability to convert fatty acids into alka(e)nes using merely visible blue light. Unfortunately, there are still some drawbacks that limit the applicability of this biocatalyst, such as poor solubility of the substrates in aqueous media, poor photostability, and the impossibility of reusing the catalyst for several cycles. In this work, we demonstrate the use of FAP in non-conventional media as a free enzyme and an immobilized preparation. Namely, its applicability in deep eutectic solvents (DESs) and a proof-of-concept immobilization using a commercial His-tag selective carrier, a thorough study of reaction and immobilization conditions in each case, as well as reu

SUBMITTER: Chanquia SN 

PROVIDER: S-EPMC10099500 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature