Genetic and physiological insights into the diazotrophic activity of a non-cyanobacterial marine diazotroph.

ABSTRACT: Nitrogen (N₂ ) fixation, or diazotrophy, supports a large part of primary production in oceans. Culture-independent approaches highlighted the presence in abundance of marine non-cyanobacterial diazotrophs (NCD), but their ecophysiology remains elusive, mostly because of the low number of isolated NCD and because of the lack of available genetic tools for these isolates. Here, a dual genetic and functional approach allowed unveiling the ecophysiology of a marine NCD affiliated to the species Vibrio diazotrophicus. Physiological characterization of the first marine NCD mutant obtained so far was performed using a soft-gellan assay, demonstrating that a ΔnifH mutant is not able to grow in nitrogen-free media. Furthermore, we demonstrated that V. diazotrophicus produces a thick bio

SUBMITTER: Joublin-Delavat A 

PROVIDER: S-EPMC10099842 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature