In vitro and in vivo gene transfer in the cloudy catshark Scyliorhinus torazame.

ABSTRACT: Cartilaginous fishes have various unique physiological features such as a cartilaginous skeleton and a urea-based osmoregulation strategy for adaptation to their marine environment. Also, because they are a sister group of bony vertebrates, understanding their unique features is important from an evolutionary perspective. However, genetic engineering based on gene functions as well as cellular behavior has not been effectively utilized in cartilaginous fishes. This is partly because their reproductive strategy involves internal fertilization, which results in difficulty in microinjection into fertilized eggs at the early developmental stage. Here, to identify efficient gene transfer methods in cartilaginous fishes, we examined the effects of various methods both in vitro and in vivo using

SUBMITTER: Fujimori C 

PROVIDER: S-EPMC10099843 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature