Probing Cell-Surface Interactions in Fungal Cell Walls by High-Resolution ¹ H-Detected Solid-State NMR Spectroscopy.

ABSTRACT: Solid-state NMR (ssNMR) spectroscopy facilitates the non-destructive characterization of structurally heterogeneous biomolecules in their native setting, for example, comprising proteins, lipids and polysaccharides. Here we demonstrate the utility of high and ultra-high field ¹ H-detected fast MAS ssNMR spectroscopy, which exhibits increased sensitivity and spectral resolution, to further elucidate the atomic-level composition and structural arrangement of the cell wall of Schizophyllum commune, a mushroom-forming fungus from the Basidiomycota phylum. These advancements allowed us to reveal that Cu(II) ions and the antifungal peptide Cathelicidin-2 mainly bind to cell wall proteins at low concentrations while glucans are targeted at high metal ion concentrations. In addition, ou

SUBMITTER: Safeer A 

PROVIDER: S-EPMC10099940 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature