High-Resolution 3D Genome Map of Brucella Chromosomes in Exponential and Stationary Phases.

ABSTRACT: The three-dimensional (3D) genome structure of an organism or cell is highly relevant to its biological activities, but the availability of 3D genome information for bacteria, especially intracellular pathogens, is still limited. Here, we used Hi-C (high-throughput chromosome conformation capture) technology to determine the 3D chromosome structures of exponential- and stationary-phase Brucella melitensis at a 1-kb resolution. We observed that the contact heat maps of the two B. melitensis chromosomes contain a prominent diagonal and a secondary diagonal. Then, 79 chromatin interaction domains (CIDs) were detected at an optical density at 600 nm (OD₆₀₀) of 0.4 (exponential phase), with the longest CID being 106 kb and the shortest being 12 kb. Moreover, we obtained 49,363 signif

SUBMITTER: Huang YF 

PROVIDER: S-EPMC10100373 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature