Nested PCR Approach for petB Gene Metabarcoding of Marine Synechococcus Populations.

ABSTRACT: The molecular diversity of marine picocyanobacterial populations, an important component of phytoplankton communities, is better characterized using high-resolution marker genes than the 16S rRNA gene as they have greater sequence divergence to differentiate between closely related picocyanobacteria groups. Although specific ribosomal primers have been developed, another general disadvantage of bacterial ribosome-based diversity analyses is the variable number of rRNA gene copies. To overcome these issues, the single-copy petB gene, encoding the cytochrome b₆ subunit of the cytochrome b₆f complex, has been used as a high-resolution marker gene to characterize Synechococcus diversity. We have designed new primers targeting the petB

SUBMITTER: Ong DRY 

PROVIDER: S-EPMC10100653 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature