Spontaneous Genomic Variation as a Survival Strategy of Nosocomial Staphylococcus haemolyticus.

ABSTRACT: Staphylococcus haemolyticus is one of the most important nosocomial human pathogens frequently isolated in bloodstream and medical device-related infections. However, its mechanisms of evolution and adaptation are still poorly explored. To characterize the strategies of genetic and phenotypic diversity in S. haemolyticus, we analyzed an invasive strain for genetic and phenotypic stability after serial passage in vitro in the absence and presence of beta-lactam antibiotics. We performed pulsed-field gel electrophoresis (PFGE) of the culture and analyzed five colonies at seven time points during stability assays for beta-lactam susceptibility, hemolysis, mannitol fermentation, and biofilm production. We compared their whole genomes and performed phylogenetic analysis based on c

SUBMITTER: Bouchami O 

PROVIDER: S-EPMC10100732 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature