Intra-Domain Residue Coevolution in Transcription Factors Contributes to DNA Binding Specificity.

ABSTRACT: Understanding the basis of the DNA-binding specificity of transcription factors (TFs) has been of long-standing interest. Despite extensive efforts to map millions of putative TF binding sequences, identifying the critical determinants for DNA binding specificity remains a major challenge. The coevolution of residues in proteins occurs due to a shared evolutionary history. However, it is unclear how coevolving residues in TFs contribute to DNA binding specificity. Here, we systematically collected publicly available data sets from multiple large-scale high-throughput TF-DNA interaction screening experiments for the major TF families with large numbers of TF members. These families included the Homeobox, HLH, bZIP_1, Ets, HMG_box, ZF-C4, and Zn_clus TFs. We detected TF subclass-determining

SUBMITTER: Luan Y 

PROVIDER: S-EPMC10100741 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature