In Vitro Modelling of Oral Microbial Invasion in the Human Colon.

ABSTRACT: Recent advances in the human microbiome characterization have revealed significant oral microbial detection in stools of dysbiotic patients. However, little is known about the potential interactions of these invasive oral microorganisms with commensal intestinal microbiota and the host. In this proof-of-concept study, we proposed a new model of oral-to-gut invasion by the combined use of an in vitro model simulating both the physicochemical and microbial (lumen- and mucus-associated microbes) parameters of the human colon (M-ARCOL), a salivary enrichment protocol, and whole-metagenome shotgun sequencing. Oral invasion of the intestinal microbiota was simulated by injection of enriched saliva in the in vitro colon model inoculated with a fecal sample from the same healthy adul

SUBMITTER: Etienne-Mesmin L 

PROVIDER: S-EPMC10100946 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature