Rapid Detection of Staphylococcus aureus in Milk and Pork via Immunomagnetic Separation and Recombinase Polymerase Amplification.

ABSTRACT: Separation processes using immunomagnetic beads (IMBs) are advantageous for the rapid detection of Staphylococcus aureus (S. aureus). Herein, a novel method, based on immunomagnetic separation using IMBs and recombinase polymerase amplification (RPA), was employed to detect S. aureus strains in milk and pork. IMBs were formed by the carbon diimide method using rabbit anti-S. aureus polyclonal antibodies and superparamagnetic carboxyl-Fe₃O₄ MBs. The average capture efficiency for 2.5 to 2.5 × 10⁵ (CFU)/mL gradient dilution of S. aureus with 6 mg of IMBs within 60 min were a range of 62.74 to 92.75%. The detection sensitivity of the IMBs-RPA method in artificially contaminated samples was 2.5 × 10¹ CFU/mL. The entire detection process was completed

SUBMITTER: Cheng R 

PROVIDER: S-EPMC10101137 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature