Reducing bias in microbiome research: Comparing methods from sample collection to sequencing.

ABSTRACT:

Background

Microbiota profiles are strongly influenced by many technical aspects that impact the ability of researchers to compare results. To investigate and identify potential biases introduced by technical variations, we compared several approaches throughout the entire workflow of a microbiome study, from sample collection to sequencing, using commercially available mock communities (from bacterial strains as well as from DNA) and multiple human fecal samples, including a large set of positive controls created as a random mix of several participant samples.

Methods

Human fecal material was sampled, and aliquots were used to test two commercially available stabilization solutions (OMNIgene·GUT and Zymo Research) in comparison to samples frozen immediately upon collection.

SUBMITTER: Kool J 

PROVIDER: S-EPMC10101209 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature