L1CAM immunocapture generates a unique extracellular vesicle population with a reproducible miRNA fingerprint.

ABSTRACT: Micro RNAs (miRNAs) are short, non-coding RNAs with significant potential as diagnostic and prognostic biomarkers. However, a lack of reproducibility across studies has hindered their introduction into clinical settings. Inconsistencies between studies include a lack of consensus on the miRNAs associated with a specific disease and the direction of regulation. These differences may reflect the heterogenous nature of pathologies with multiple phenotypes, such as amyotrophic lateral sclerosis (ALS). It is also possible that discrepancies are due to different sampling, processing, and analysis protocols across labs. Using miRNA extracted from L1CAM immunoaffinity purified extracellular vesicles (neural-enriched extracellular vesicles or NEE), we thrice replicated an 8-miRNA fingerprint diagno

SUBMITTER: Dunlop RA 

PROVIDER: S-EPMC10101655 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature