Storage stability and HILIC-UHPLC-FLR analysis of immunoglobulin G N-glycome from saliva.

ABSTRACT: Immunoglobulin G (IgG) is the most abundant antibody in the blood and plays a critical role in host immune defense against infectious agents. Glycosylation is known to modulate the effector functions of IgG and is involved in disease development and progression. It is no surprise that the N-glycome of IgG from plasma has already been proposed as a biomarker for various physiological and pathological conditions. However, because saliva is easy to collect, it could be useful for exploring the functional role of salivary IgG N-glycosylation and its potential as a diagnostic biomarker. Therefore, in this study, we described a method for N-glycome analysis of IgG from saliva samples. Salivary IgG N-glycans were analyzed by ultra-high-performance liquid chromatography based on hydrophilic intera

SUBMITTER: Radovani B 

PROVIDER: S-EPMC10101819 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature