Epigenetic and genetic inactivation of tumor suppressor miR-135a in non-small-cell lung cancer.

ABSTRACT:

Background

Despite therapeutic advances, lung cancer prognosis remains poor. Loss of heterozygosity (LOH) in the 3p21 region is well documented in lung cancer, but the specific causative genes have not been identified.

Materials and methods

Here, we aimed to examine the clinical impact of miR-135a, located in the 3p21 region, in lung cancer. miR-135a expression was assessed using quantitative real-time polymerase chain reaction. LOH was analyzed at microsatellite loci D3S1076 and D3S1478, and promoter methylation status was determined by pyrosequencing of resected samples of primary non-small-cell lung cancer (NSCLC). The regulation of telomerase reverse transcriptase (TERT) was evaluated in lung cancer cells H1299 by luciferase report assays after treatment with miR-135a mi

SUBMITTER: Choi JE 

PROVIDER: S-EPMC10101835 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature