Increasing cell culture density during a developmental window prevents fated rod precursors derailment toward hybrid rod-glia cells.

ABSTRACT: In proliferating multipotent retinal progenitors, transcription factors dynamics set the fate of postmitotic daughter cells, but postmitotic cell fate plasticity driven by extrinsic factors remains controversial. Transcriptome analysis reveals the concurrent expression by postmitotic rod precursors of genes critical for the Müller glia cell fate, which are rarely generated from terminally-dividing progenitors as a pair with rod precursors. By combining gene expression and functional characterisation in single cultured rod precursors, we identified a time-restricted window where increasing cell culture density switches off the expression of genes critical for Müller glial cells. Intriguingly, rod precursors in low cell culture density maintain the expression of genes of rod and glial cell f

SUBMITTER: Barravecchia I 

PROVIDER: S-EPMC10101963 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature