Single cell transcriptomics reveals reduced stress response in stem cells manipulated using localized electric fields.

ABSTRACT: Membrane disruption using Bulk Electroporation (BEP) is a widely used non-viral method for delivering biomolecules into cells. Recently, its microfluidic counterpart, Localized Electroporation (LEP), has been successfully used for several applications ranging from reprogramming and engineering cells for therapeutic purposes to non-destructive sampling from live cells for temporal analysis. However, the side effects of these processes on gene expression, that can affect the physiology of sensitive stem cells are not well understood. Here, we use single cell RNA sequencing (scRNA-seq) to investigate the effects of BEP and LEP on murine neural stem cell (NSC) gene expression. Our results indicate that unlike BEP, LEP does not lead to extensive cell death or activation of cell st

SUBMITTER: Mukherjee P 

PROVIDER: S-EPMC10102005 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature