Mechanical stimulation controls osteoclast function through the regulation of Ca²⁺-activated Cl^- channel Anoctamin 1.

ABSTRACT: Mechanical force loading is essential for maintaining bone homeostasis, and unloading exposure can lead to bone loss. Osteoclasts are the only bone resorbing cells and play a crucial role in bone remodeling. The molecular mechanisms underlying mechanical stimulation-induced changes in osteoclast function remain to be fully elucidated. Our previous research found Ca²⁺-activated Cl^- channel Anoctamin 1 (Ano1) was an essential regulator for osteoclast function. Here, we report that Ano1 mediates osteoclast responses to mechanical stimulation. In vitro, osteoclast activities are obviously affected by mechanical stress, which is accompanied by the changes of Ano1 levels, intracellular Cl^- concentration and Ca²⁺ downstream signaling. Ano1 knockout or c

SUBMITTER: Sun W 

PROVIDER: S-EPMC10102170 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature