Small-Molecule Aptamer for Regulating RNA Functions in Mammalian Cells and Animals.

ABSTRACT: Synthetic riboswitches that can regulate gene expression by a small molecule recognized by an RNA aptamer in mammalian cells have various potential applications in biotechnology and medicine. However, the variety of small molecules and their cognate aptamers that have been demonstrated to function in mammalian cells is limited. The currently available aptamer-ligand pairs also require high small molecule concentrations to enable gene regulation, making them less desirable for industrial and biomedical applications. We conducted in vitro selection of RNA aptamers against a small molecule ASP7967 whose structure is closely related to ASP2905, a known inhibitor of potassium voltage-gated channel sub-family H member 3 (KCNH3). One of the aptamers selected (AC17-4) was found to be functi

SUBMITTER: Fukunaga K 

PROVIDER: S-EPMC10103173 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature