Single-cell long-read mRNA isoform regulation is pervasive across mammalian brain regions, cell types, and development.

ABSTRACT: RNA isoforms influence cell identity and function. Until recently, technological limitations prevented a genome-wide appraisal of isoform influence on cell identity in various parts of the brain. Using enhanced long-read single-cell isoform sequencing, we comprehensively analyze RNA isoforms in multiple mouse brain regions, cell subtypes, and developmental timepoints from postnatal day 14 (P14) to adult (P56). For 75% of genes, full-length isoform expression varies along one or more axes of phenotypic origin, underscoring the pervasiveness of isoform regulation across multiple scales. As expected, splicing varies strongly between cell types. However, certain gene classes including neurotransmitter release and reuptake as well as synapse turnover, harbor significant variability in the same

SUBMITTER: Joglekar A 

PROVIDER: S-EPMC10103983 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature