Pulse train gating to improve signal generation for in vivo two-photon fluorescence microscopy.

ABSTRACT:

Significance

Two-photon microscopy is used routinely for in vivo imaging of neural and vascular structure and function in rodents with a high resolution. Image quality, however, often degrades in deeper portions of the cerebral cortex. Strategies to improve deep imaging are therefore needed. We introduce such a strategy using gates of high repetition rate ultrafast pulse trains to increase signal level.

Aim

We investigate how signal generation, signal-to-noise ratio (SNR), and signal-to-background ratio (SBR) improve with pulse gating while imaging in vivo mouse cerebral vasculature.

Approach

An electro-optic modulator is used with a high-power (6 W) 80 MHz repetition rate ytterbium fiber amplifier to create gates of pulses at a 1 MHz repetition rate. We

SUBMITTER: Engelmann SA 

PROVIDER: S-EPMC10103994 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature