Stepwise design of pseudosymmetric protein hetero-oligomers.

ABSTRACT: Pseudosymmetric hetero-oligomers with three or more unique subunits with overall structural (but not sequence) symmetry play key roles in biology, and systematic approaches for generating such proteins de novo would provide new routes to controlling cell signaling and designing complex protein materials. However, the de novo design of protein hetero-oligomers with three or more distinct chains with nearly identical structures is a challenging problem because it requires the accurate design of multiple protein-protein interfaces simultaneously. Here, we describe a divide-and-conquer approach that breaks the multiple-interface design challenge into a set of more tractable symmetric single-interface redesign problems, followed by structural recombination of the validated homo-ol

SUBMITTER: Kibler RD 

PROVIDER: S-EPMC10104133 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature