Differentially expressed transcript isoforms associate with resistance to tuberculin skin test and interferon gamma release assay conversion.

ABSTRACT:

Background

A mechanistic understanding of uncommon immune outcomes such as resistance to infection has led to the development of novel therapies. Using gene level analytic methods, we previously found distinct monocyte transcriptional responses associated with resistance to Mycobacterium tuberculosis (Mtb) infection defined as persistently negative tuberculin skin test (TST) and interferon gamma release assay (IGRA) reactivity among highly exposed contacts (RSTR phenotype).

Objective

Using transcript isoform analyses, we aimed to identify novel RSTR-associated genes hypothesizing that previous gene-level differential expression analysis obscures isoform-specific differences that contribute to phenotype.

Materials and methods

Monocytes from 49 RSTR versus 52 subjects w

SUBMITTER: Simmons JD 

PROVIDER: S-EPMC10104279 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature