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The linkage between prevalence of integron I and reduced susceptibility to biocides in MDR Klebsiella pneumoniae isolated from neonates.

ABSTRACT:

Background and objectives

Klebsiella pneumoniae causes challenging nosocomial fatal infections including neonatal sepsis. Our study aims at clarifying the contribution of integrons in the observed reduced susceptibility of multidrug-resistant (MDR) K. pneumoniae isolated from septicemic neonates to the clinically used antimicrobial agents and biocides.

Materials and methods

Eighty-six K. pneumoniae isolates were collected from Mansoura University Children's Hospital from septicemic neonates. Isolates were subjected to antibiotic and biocide susceptibility using disk diffusion and the agar dilution method, respectively. The distribution of different classes of integrons was screened in the isolates by PCR. Detected inegron I was sequenced in selected isolates.

Results

Fifty-seven isolates (66.27%) were MDR. In the MDR isolates, class I integron was detected in 23 (40.3%), integron III was detected in 20 (35%), whereas integron II could not be detected. Sequencing results of integron I from MDR K. pneumoniae isolates revealed that only aminoglycoside and folate synthesis inhibitors gene cassettes were detected, while the rest of the resistance genes were not associated with integron I.

Conclusion

The presence of integron I in MDR K. pneumoniae tested isolates may contribute only to some biocide resistance, however, it does not seem to be the only contributor in multiple drug resistance.

SUBMITTER: Samir P

PROVIDER: S-EPMC10105274 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature

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The linkage between prevalence of integron I and reduced susceptibility to biocides in MDR <i>Klebsiella pneumoniae</i> isolated from neonates.

Samir Pola P El-Baz Ahmed Mohamed AM Kenawy Hany Ibrahim HI

Iranian journal of microbiology 20230201 1

<h4>Background and objectives</h4><i>Klebsiella pneumoniae</i> causes challenging nosocomial fatal infections including neonatal sepsis. Our study aims at clarifying the contribution of integrons in the observed reduced susceptibility of multidrug-resistant (MDR) <i>K. pneumoniae</i> isolated from septicemic neonates to the clinically used antimicrobial agents and biocides.<h4>Materials and methods</h4>Eighty-six <i>K. pneumoniae</i> isolates were collected from Mansoura University Children's Ho ...[more]

PMID: 37069917

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