A novel method for visualizing in-vivo rates of protein degradation provides insight into how TRIM28 regulates muscle size.

ABSTRACT: Skeletal muscle size is controlled by the balance between protein synthesis and protein degradation. Given the essential role of skeletal muscle in maintaining a high quality of life, understanding the mechanisms that modulate this balance are of critical importance. Previously, we demonstrated that muscle-specific knockout of TRIM28 reduces muscle size and function and in the current study, we discovered that this effect is associated with an increase in protein degradation and a dramatic reduction in the expression of Mettl21c. Importantly, we also determined that overexpression of Mettl21c is sufficient to induce hypertrophy in both control and TRIM28 knockout muscles. Moreover, we developed a simple pulse-chase biorthogonal non-canonical amino acid tagging technique that enabled us to

SUBMITTER: Steinert ND 

PROVIDER: S-EPMC10105291 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature