Caffeine activates HOG-signalling and inhibits pseudohyphal growth in Saccharomyces cerevisiae.

ABSTRACT:

Objective

Caffeine has a wide range of effects in humans and other organisms. Caffeine activates p38 MAPK, the human homolog to the Hog1 protein that orchestrates the high-osmolarity glycerol (HOG) response to osmotic stress in the yeast Saccharomyces cerevisiae. Caffeine has also been used as an inducer of cell-wall stress in yeast via its activation of the Pkc1-mediated cell wall integrity (CWI) pathway. In this study, using immunodetection of phosphorylated Hog1, microscopy to score nuclear localisation of GFP-tagged Hog1 and a pseudohyphal growth assays, the effect of caffeine on the HOG-pathway and filamentous growth in yeast was studied.

Results

It was found that caffeine causes rapid, strong and transient Hog1 dual phosphorylation with statistically significant increa

SUBMITTER: Elhasi T 

PROVIDER: S-EPMC10105414 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature