Exploring the potential application of alternative nuclei in NMR based metabolomics.

ABSTRACT:

Introduction

Nuclear magnetic resonance (NMR) is widely used in metabolomics but it focusses on ¹H over other NMR-active nuclei.

Objectives

To evaluate the potential of alternative NMR-sensitive nuclei to generate useful metabolomic data.

Method

Proton, carbon, phosphorus, and nitrogen-based NMR metabolomics was undertaken on extracts from mint and European honey bee tissue.

Results

Carbon NMR provided useful information but required larger sample sizes. Phosphorus produced overlapping peaks in one dimensional (1D) analysis but showed potential in 2D experiments. ¹⁵N NMR was found to not be sensitive enough for general metabolomic work.

Conclusions

Alternative NMR active nuclei are useful for metabolomics.

SUBMITTER: Sinclair GM 

PROVIDER: S-EPMC10105680 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature