Ligand sensitivity of type-1 inositol 1,4,5-trisphosphate receptor is enhanced by the D2594K mutation.

ABSTRACT: Inositol 1,4,5-trisphosphate receptor (IP₃R) and ryanodine receptor (RyR) are homologous cation channels that mediate release of Ca²⁺ from the endoplasmic/sarcoplasmic reticulum (ER/SR) and thereby are involved in many physiological processes. In previous studies, we determined that when the D2594 residue, located at or near the gate of the IP₃R type 1, was replaced by lysine (D2594K), a gain of function was obtained. This mutant phenotype was characterized by increased IP₃ sensitivity. We hypothesized the IP₃R1-D2594 determines the ligand sensitivity of the channel by electrostatically affecting the stability of the closed and open states. To test this possibility, the relationship between the D2594 site and IP₃R1 regulatio

SUBMITTER: Tambeaux A 

PROVIDER: S-EPMC10105685 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature