A proof-of-concept assay for quantitative and optical assessment of drug-induced toxicity in renal organoids.

ABSTRACT: Kidneys are complex organs, and reproducing their function and physiology in a laboratory setting remains difficult. During drug development, potential compounds may exhibit unexpected nephrotoxic effects, which imposes a significant financial burden on pharmaceutical companies. As a result, there is an ongoing need for more accurate model systems. The use of renal organoids to simulate responses to nephrotoxic insults has the potential to bridge the gap between preclinical drug efficacy studies in cell cultures and animal models, and the stages of clinical trials in humans. Here we established an accessible fluorescent whole-mount approach for nuclear and membrane staining to first provide an overview of the organoid histology. Furthermore, we investigated the potential of renal organoids

SUBMITTER: Dilz J 

PROVIDER: S-EPMC10105743 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature