Robustness and reproducibility of simple and complex synthetic logic circuit designs using a DBTL loop.

ABSTRACT: Computational tools addressing various components of design-build-test-learn (DBTL) loops for the construction of synthetic genetic networks exist but do not generally cover the entire DBTL loop. This manuscript introduces an end-to-end sequence of tools that together form a DBTL loop called Design Assemble Round Trip (DART). DART provides rational selection and refinement of genetic parts to construct and test a circuit. Computational support for experimental process, metadata management, standardized data collection and reproducible data analysis is provided via the previously published Round Trip (RT) test-learn loop. The primary focus of this work is on the Design Assemble (DA) part of the tool chain, which improves on previous techniques by screening up to thousands of network topolog

SUBMITTER: Cummins B 

PROVIDER: S-EPMC10105856 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature