Discovery of novel amino acid production traits by evolution of synthetic co-cultures.

ABSTRACT:

Background

Amino acid production features of Corynebacterium glutamicum were extensively studied in the last two decades. Many metabolic pathways, regulatory and transport principles are known, but purely rational approaches often provide only limited progress in production optimization. We recently generated stable synthetic co-cultures, termed Communities of Niche-optimized Strains (CoNoS), that rely on cross-feeding of amino acids for growth. This setup has the potential to evolve strains with improved production by selection of faster growing communities.

Results

Here we performed adaptive laboratory evolution (ALE) with a CoNoS to identify mutations that are relevant for amino acid production both in mono- and co-cultures. During ALE with the CoNoS composed of strains a

SUBMITTER: Zuchowski R 

PROVIDER: S-EPMC10105947 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature