Telomerase-mediated immortalization of human vaginal wall fibroblasts derived from patients with pelvic organ prolapse.

ABSTRACT:

Background

Extracellular matrix (ECM) remodeling is the most important pathomechanism of pelvic organ prolapse (POP). Fibroblasts are the key to ECM regulation. The passaging capacity of human vaginal wall fibroblasts (hVWFs) is limited in vitro . Here, we aimed to immortalize hVWFs through the introduction of human telomerase reverse transcriptase (hTERT).

Methods

Primary cells were derived from the vaginal wall tissue of patients with POP. Cellular senescence was detected via senescence-associated β-galactosidase staining. We employed a lentiviral transfection vector to stably express hTERT in hVWFs at passage 3, generating immortalized hVWFs (i-hVWFs). We then assessed cellular proliferation via the CCK-8 and EdU assays as well as cellular migration via wound healing assa

SUBMITTER: Guo T 

PROVIDER: S-EPMC10106167 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature