Microglial reprogramming by Hv1 antagonism protects neurons from inflammatory and glutamate toxicity.

ABSTRACT: Although the precise mechanisms determining the neurotoxic or neuroprotective activation phenotypes in microglia remain poorly characterized, metabolic changes in these cells appear critical for these processes. As cellular metabolism can be tightly regulated by changes in intracellular pH, we tested whether pharmacological targeting of the microglial voltage-gated proton channel 1 (Hv1), an important regulator of intracellular pH, is critical for activated microglial reprogramming. Using a mouse microglial cell line and mouse primary microglia cultures, either alone, or co-cultured with rat cerebrocortical neurons, we characterized in detail the microglial activation profile in the absence and presence of Hv1 inhibition. We observed that activated microglia neurotoxicity was mainly attrib

SUBMITTER: Hernandez-Espinosa DR 

PROVIDER: S-EPMC10106429 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature