Understanding the potential immunogenetic role of TNFα-308 polymorphism in the pathogenesis of recurrent miscarriage.

ABSTRACT:

Background

Recurrent miscarriage (RM) represents the spontaneous termination of two or more successive pregnancies. TNFα is a proinflammatory cytokine that is often considered harmful for embryonic development when expressed beyond normal levels.

Aim

The study was conducted to assess the association between TNFα-308 polymorphism and RM pathogenesis.

Methods

Samples of blood were obtained from patients and controls through venipuncture. The levels of TNFα in serum were measured by ELISA. TNFα gene promoter-associated single-nucleotide polymorphism was investigated with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) techniques with precise primers and the restriction endonuclease, NcoI.

Results

Serum TNFα levels in patients we

SUBMITTER: Ali S 

PROVIDER: S-EPMC10106509 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature