RNA-seq analysis of synchronized developing pollen isolated from a single anther.

ABSTRACT: Pollen development, from unicellular microspores to anthesis, is a complex process involving the coordinated specification, differentiation and functions of different cell types. Key to understanding this development is identifying the genes expressed at precise stages of development. However, transcriptomic studies on pollen prior to anthesis are complicated by the inaccessible nature of pollen developing in the anther and the resistant pollen wall. To assist with understanding gene expression during pollen development we have developed a protocol to perform RNA-Seq on pollen isolated from a single anther (SA RNA-Seq). The protocol involves removing pollen from a single anther for analysis and viewing the remaining pollen to determine the developmental stage. The isolated pollen is chemic

SUBMITTER: Le Lievre L 

PROVIDER: S-EPMC10106640 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature