Development and assessment of a novel gold immunochromatographic assay for the diagnosis of schistosomiasis japonica.

ABSTRACT:

Background

The neglected zoonosis, schistosomiasis japonica, remains a major public health problem in the Philippines. The current study aims to develop a novel gold immunochromatographic assay (GICA) and evaluate its performance in the detection of Schistosoma japonicum infection.

Methods

A GICA strip incorporating a S. japonicum saposin protein, SjSAP4 was developed. For each GICA strip test, diluted serum sample (50 µl) was loaded and strips were scanned after 10 min to convert the results into images. ImageJ was used to calculate an R value, which was defined as the signal intensity of the test line divided by the signal intensity of the control line within the cassette. After determination of optimal serum dilution and diluent, the GICA assay was evaluated

SUBMITTER: Mu Y 

PROVIDER: S-EPMC10106775 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature