The C₂ H₂ -type zinc finger transcription factor OSIC1 positively regulates stomatal closure under osmotic stress in poplar.

ABSTRACT: Salt and drought impair plant osmotic homeostasis and greatly limit plant growth and development. Plants decrease stomatal aperture to reduce water loss and maintain osmotic homeostasis, leading to improved stress tolerance. Herein, we identified the C₂ H₂ transcription factor gene OSMOTIC STRESS INDUCED C₂ H₂ 1 (OSIC1) from Populus alba var. pyramidalis to be induced by salt, drought, polyethylene glycol 6000 (PEG6000) and abscisic acid (ABA). Overexpression of OSIC1 conferred transgenic poplar more tolerance to high salinity, drought and PEG6000 treatment by reducing stomatal aperture, while its mutant generated by the CRISPR/Cas9 system showed the opposite phenotype. Furthermore, OSIC1 directly up-regulates PalCuAOζ in vitro and in vivo, encod

SUBMITTER: Bai Q 

PROVIDER: S-EPMC10106854 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature