Adaptation to high rates of chromosomal instability and aneuploidy through multiple pathways in budding yeast.

ABSTRACT: Both an increased frequency of chromosome missegregation (chromosomal instability, CIN) and the presence of an abnormal complement of chromosomes (aneuploidy) are hallmarks of cancer. To better understand how cells are able to adapt to high levels of chromosomal instability, we previously examined yeast cells that were deleted of the gene BIR1, a member of the chromosomal passenger complex (CPC). We found bir1Δ cells quickly adapted by acquiring specific combinations of beneficial aneuploidies. In this study, we monitored these yeast strains for longer periods of time to determine how cells adapt to high levels of both CIN and aneuploidy in the long term. We identify suppressor mutations that mitigate the chromosome missegregation phenotype. The mutated proteins fall into four main categor

SUBMITTER: Clarke MN 

PROVIDER: S-EPMC10106982 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature