Distinct mechanisms of CB1 and GABA_B receptor presynaptic modulation of striatal indirect pathway projections to mouse globus pallidus.

ABSTRACT: Presynaptic modulation is a fundamental process regulating synaptic transmission. Striatal indirect pathway projections originate from A2A-expressing spiny projection neurons (iSPNs), targeting the globus pallidus external segment (GPe) and control the firing of the tonically active GPe neurons via GABA release. It is unclear if and how the presynaptic G-protein-coupled receptors (GPCRs), GABA_B and CB1 receptors modulate iSPN-GPe projections. Here we used an optogenetic platform to study presynaptic Ca²⁺ and GABAergic transmission at iSPN projections, using a genetic strategy to express the calcium sensor GCaMP6f or the excitatory channelrhodopsin (hChR2) on iSPNs. We found that P/Q-type calcium channels are the primary voltage-gated Ca²⁺ channel (VGCC) sub

SUBMITTER: Sitzia G 

PROVIDER: S-EPMC10107704 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature